Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.4 (0.2 micron filtered), 500 ml.
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link:
eMolecules Building Block Tool<|a>
Quality Biological Inc TAE Buffer 50X, 10 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC4020577 ELUTE BUFFER B 50MM TRIS HCL8
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5ML
NC3588252 50 MM TRIS-HCL 10 MM EDTA 5ML
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTAPH8
NC3588273 50 MM TRIS-HCL 10 MM EDTAPH8
Bioworld Bis-Tris Propane, 1 kg
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5
Bioworld Tris-Acetate-SDS Running Buffer (10X), 500 mL
TRIS-Acetate-SDS Running Buffer (10X) All bioWORLD running buffers are prepared with high quality reagents. Technical Information:Dilute with deionized water to make 1X. 10X concentration1X contains: 50mM Tris base, 50mM Tricine, 3.5mM SDS pH 8.24
Bioworld Tris-Glycine Sample Buffer (4X), 50 mL
Product DescriptionTris-Glycine (TG) sample buffer has a concentration of 4X. It contains 400 mM Tris-HCl at a pH of 8.6, 40% glycerol, and 0.02% bromophenol blue.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Medchemexpress LLC 1H-1,4,7-triazonine-1,4,7-triacetic acid, α1-(2-carboxyethyl)hexahydro-, tris(t-Bu) ester | 1190101-34-8 | MFCD29083820 | 99.8% | 543.69 g/mol | C27H49N3O8 | 100 MG
NODAGA-tris(t-Bu ester) is a tri-tert-butyl ester-protected NODAGA chelator intended for conjugation to biomolecules and subsequent radiolabeling for positron emission tomography (PET) studies. It provides a protected, bifunctional scaffold that facilitates attachment to peptides, proteins, and antibodies and enables stable chelation of radiometals after deprotection.
- Protected NODAGA chelator for conjugation to biomolecules.
- Suitable for labeling peptides, proteins, and antibodies.
- Designed for radiolabeling workflows for PET imaging.
- High purity (99.8%) with RP-HPLC documentation available.
- Available as solid and as DMSO solutions for convenience.
- Molecular formula C27H49N3O8; molecular weight 543.69 g/mol.