Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TAE Buffer, Quick-Mix Pack (10X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 10X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Crescent Chemical Co Inc TBE BUFFER (10X)
TBE Buffer (10x) 10 x concentrated aqueous solution.TBE Buffer is widely used for the electrophoresis of nucleic acids and has a higher buffer capacity than TAE. It can be used for DNA and RNA polyacrylamide and agarose gel electrophoresis. Size - 2x500ML
![Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31022-500.03.JPG-250.jpg)
Research Products International Corp TES, Free Acid [2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid], 500 Grams
Substance Name: 2-Tris(hydroxymethyl)-methyl-2-amino 1-ethanesulfonic acid
CAS Number: 7365-44-8
Molecular Formula: C6H15NO6S
Molecular Weight: 229.25 g/mol
Boston Bioproducts Inc Tris-EDTA Buffer, TE (20X, pH 8.0) - 250ml
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of a 20X concentration of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allows pH regulation within the range of 7.5-8.5. It is widely used often for DNA and RNA gel electrophoresis, and also for DNA automated sequencing.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4, RNase Free) - 250ml
Tris-HCl Buffer (1 M pH 7 4 RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Teknova 100X TE Buffer, pH 8.0. 4L, Sterile, DNase/RNase Tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 1M Tris-HCl, pH 7.4. 100mL. Sterile 10 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Revvity Health Sciences Inc HTRF PPI Terbium detection buffer, 220 ml
HTRF PPI Terbium detection buffer, 220 ml
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.8) - 1L
Tris-HCl Buffer (1 M pH 7 8) - 1L (For Research Use and Further Manufacturing Only)
Teknova 1M Tris-HCl, pH 8.0 Solution, 1000mL, Sterile. 2 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Biotang Inc Tris-Glycine-SDS buffer (10X), 2000ml, Gel Running buffer
Tris-Glycine-SDS buffer (10X), 2000ml, Gel Running buffer.
Chemscene ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 100g
BIS-TRIS | Purity: ≥99.0% (NT) | MW:209.24 | 6976-37-0 | MFCD00002853 | 100g